Jubei-chan 2: The Counter Attack of Siberian Yagyu (十兵衛ちゃん２ ～シベリア柳生の逆襲～ Jubei-chan 2 ~Siberia Yagyuu no Gyakushuu~) is a 13-episode anime series written and directed by Akitaro Daichi, like its prequel Jubei-chan. It is animated by the same company, Madhouse Production. Jubei-chan 2 aired in the year 2004 in Japan and has been released in North America by Geneon Entertainment.

The second series is more serious than the prequel, but still relies heavily on humor while maintaining drama. The story focuses on the same protagonist, Jiyu Nanohana, a teenage girl who transforms into Jubei Yagyu the Second while wearing the Lovely Eyepatch, an eye patch consisting of a polished pink heart-shaped stone.

300 years ago, the Northern Yagyu were driven out of Japan into Siberia by the Edo Yagyu. After living several years in Siberia, they changed their name to the Siberian Yagyu. The leader of the Edo Yagyu sent Jubei Yagyu to assassinate the head of the Siberian clan, Kita Ressai. Jubei did not wish to carry out this order, but traveled to Siberia anyway. During his time there, he fell in love and had a daughter named Freesia. Some time later he caught up with Kita Ressai, the leader he had been sent to assassinate. Over Jubei's objections, Kita Ressai provoked a duel between the two while on a frozen body of water. In the heat of the battle, Kita Ressai broke through the ice, drowning Jubei Yagyu's wife and daughter along with himself. Freesia remained frozen in a glacier for 300 years, until global warming caused enough of the glacier to melt to free her.

Freesia then learns of the Lovely Eyepatch that her father left to his successor. Freesia is furious when she learns that the Lovely Eyepatch was not left to her, but rather Jiyu Nanohana. Determined to claim the Lovely Eyepatch as the daughter of Jubei Yagyu, she tracks down Jiyu Nanohana, intent on defeating Jiyu and making her suffer.

At the same time, the Siberian Yagyu learn of the appearance of Jubei Yagyu the Second and track down Jiyu Nanohana in an attempt to satisfy their 300 year old grudge against Jubei Yagyu.

Now Jiyu faces two distinct enemies: the Siberian Yagyu and Freesia Yagyu.

Freesia has not forgotten what the Siberian Yagyu did to her family, either, and is determined to defeat the Siberian Yagyu after claiming the Lovely Eyepatch as her own.

A major theme of the series is power and how differently people react to it. Jiyu doesn't want the responsibility and she's afraid to lose herself (Jiyu) for power (the form of Jubei) while Freesia doesn't understand anyone who isn't willing to have more power.

From Wikipedia